The acidic environment in endocytic compartments
نویسندگان
چکیده
منابع مشابه
Intracellular fusion of sequentially formed endocytic compartments
A polyclonal anti-fluorescein antibody (AFA) which quenches fluorescein fluorescence has been used to distinguish between two models of intracellular vesicle traffic. These models address the question of whether sequentially endocytosed probes will mix intracellularly or whether they are carried through the cell in a sequential, isolated manner. Using transferrin (Tf) as a recycling receptor ma...
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A fluorescence assay developed for the quantitation of intracellular fusion of sequentially formed endocytic compartments (Salzman, N. H., and F. R. Maxfield. 1988 J. Cell Biol. 106:1083-1091) has been used to measure the time course of endosome fusion accessibility along the recycling and degradative endocytic pathways. Transferrin (Tf) was used to label the recycling pathway, and alpha2-macro...
متن کاملA view of acidic intracellular compartments
C ONSIDERABLE evidence has accumulated over the past ten years that the interior of both the endocytic and exocytic vacuolar apparatus in cells is maintained at a low pH (30, 47, 60). The interior of endocytic vesicles (4, 17, 29, 57, 59), lysosomes (37, 43), portions of the transGolgi apparatus (3, 38, 39), certain secretory vesicles (14, 22, 23, 38, 48), and plant tonoplasts (7) is acidic. Th...
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In most human and mouse antigen-presenting cells, the majority of intracellular major histocompatibility complex (MHC) class II molecules resides in late endocytic MHC class II compartments (MIICs), thought to function in antigen processing and peptide loading. However, in mouse A20 B cells, early endocytic class II-containing vesicles (CIIVs) have been reported to contain most of the intracell...
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The clinical utility of cisplatin to treat human malignancies is often limited by the development of drug resistance. We have previously shown that cisplatin-resistant human KB adenocarcinoma cells that are cross-resistant to methotrexate and heavy metals have altered endocytic recycling. In this work, we tracked lipids in the endocytic recycling compartment (ERC) and found that the distributio...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1994
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj3030679